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· Postdoc Associate in Neuroimmunology,
Miami Project to Cure Paralysis,
University of Miami, Miami, USA,
2002
· Postdoc Fellow in Molecular Biology
and Immunology, National Jewish Medical and
Research Center, University of Colorado,
Denver, USA, 1999
· Ph.D. in Neurobiology, Peking Union Medical
College (PUMC), Beijing, China, 1996
· M.S. in Traumatic Surgery, Third Military Medical University (TMMU)
, Chongqing, China, 1988
· M.D. in Medical Science, North Sichuan Medical
College (NSMC), Sichuan, China 1982
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Targeted delivery (AAV, lenvirus-like particles and nanoparticles)
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CRISPR/Cas editing and gene therapy
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Function and mechanism of NIBP/TRAPPC9 in neurodevelopmental disorders, neurodegenerative diseases and metabolic syndrome
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Exin21 boost of antibody/vaccine/virus prodution
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- Genome and epigenome editing (CRISPR/Cas)
- Signaling pathways
of NF-kB activation
- Signal transduction
of G protein-coupled receptors (GPCR)
- Neurodevelopment and adult Neurogenesis
- Small interfering
RNA (siRNA)
- Targeted AAV and Lentiviral gene
delivery
- Yeast two-hybrid
screen
- mRNA stability
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- NEB-HiFi cloning
- Co-immunoprecipitation
and Western blot
- AAV packaging and purification
- Lentivirus packaging
and purification
- Culture of iPSC and vascularized microglia-containing cerebral organoids
- Chromatin-immunoprocipitation
(CHIP)
- Protein expression and
purification
- Immunofluoresent
staining
- Real-time PCR
, droplet digital PCR
- HCR-ISH
- Dual reporter assay
- Single cell RNA-seq
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